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Tracing Pre-mRNA Processing from Transcription to a Mature mRNA

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This procedure traces a eukaryotic pre-mRNA from RNA polymerase II's transcription through capping, tailing, and splicing to a finished, translatable mRNA.

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Capping, tailing, and splicing are three separate processing events, not stages of one single reaction — a pre-mRNA missing any one of the three is not yet a mature mRNA.