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Eukaryotic RNA Polymerases I, II, and III

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Eukaryotes use three distinct RNA polymerases, of which RNA polymerase II transcribes the pre-mRNA for nearly all protein-coding genes.

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RNA polymerase I transcribes most ribosomal RNA, RNA polymerase II transcribes messenger RNA, and RNA polymerase III transcribes transfer RNA.

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Only one of the three, RNA polymerase II, transcribes protein-coding genes — the other two serve structural and catalytic RNA roles instead.