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Splicing and the Spliceosome

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Splicing removes a pre-mRNA's introns and joins its exons together, carried out by a large RNA-protein complex called the spliceosome.

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The spliceosome recognizes each intron's boundary sequences, cuts the intron out, and ligates the flanking exons directly together.

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Splicing acts only on introns identified by specific boundary sequences — it does not remove any exon sequence in the process.