Glycolysis, Overview
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Glycolysis splits one six-carbon glucose molecule into two three-carbon pyruvate molecules in the cytoplasm, netting two ATP and two NADH without oxygen.
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Nearly every living cell, aerobic or not, runs glycolysis as its first step in extracting energy from glucose.
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Glycolysis needs no oxygen and is not "anaerobic respiration" — it is the pathway every cell runs before oxygen becomes relevant.