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Biology / Unit 3   Bio 0538 short lesson, about 20 seconds

Substrate-Level Phosphorylation

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The idea

Substrate-level phosphorylation makes ATP directly, by transferring a phosphate group from a reaction intermediate straight onto ADP.

In practice

A step late in glycolysis transfers a phosphate from an intermediate molecule directly onto ADP, producing ATP without any electron transport chain.

Where people slip

Substrate-level phosphorylation is direct and immediate — it does not depend on the multi-step chemiosmotic process covered later in this unit.

Your place on the trail

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