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Recombinant Proteins

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A recombinant protein is the product of a recombinant DNA molecule once its host cell transcribes and translates the inserted gene.

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A bacterium carrying a plasmid with an inserted human gene transcribes and translates it alongside its own genes, producing the human protein.

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Inserting a gene into a plasmid supplies only the sequence — expression still needs the host's own translation machinery.