Biology / Unit 7 Bio 1535 worked example, 60 to 90 seconds
Constructing a Recombinant Plasmid
Captions available
The idea
This procedure traces building one recombinant DNA molecule, from cutting a foreign DNA fragment and a plasmid with the same restriction endonuclease through to a sealed, ligated vector.
Where people slip
Ligation only succeeds where two sticky ends are complementary — a plasmid and a fragment cut with different, non-matching restriction endonucleases will not anneal correctly.
Your place on the trail
Every lesson rests on earlier ones and opens later ones. Tap any to jump.
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