Biology / Unit 7 Bio 1579 worked example, 60 to 90 seconds
Tracing Fragments to an Assembled, Annotated Genome Sequence
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The idea
This procedure traces a genome from randomly fragmented DNA through Sanger sequencing, shotgun assembly, and annotation to a usable, function-labeled sequence.
Where people slip
Assembly produces a raw sequence; annotation is a separate, later step attaching known gene identities to it — an unannotated genome carries no labeled meaning.
Your place on the trail
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