Biology / Unit 7 Bio 1573 short lesson, about 20 seconds
Pairwise-End Sequencing
Captions available
The idea
Pairwise-end sequencing reads both ends of each DNA fragment rather than one, supplying more overlap information for reassembling larger genomes.
In practice
Knowing a fragment's start and end sequence constrains where it fits among its neighbors more tightly than knowing one end alone.
Where people slip
Pairwise-end sequencing yields more information per fragment than single-end shotgun sequencing, at the cost of more sequencing reactions.
Your place on the trail
Every lesson rests on earlier ones and opens later ones. Tap any to jump.
Something wrong in this lesson? Flag it · Sources for Biology