Biology / Unit 7 Bio 1529 short lesson, about 20 seconds
Southern and Northern Blotting
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The idea
Blotting transfers electrophoresis-separated fragments onto a membrane, then probes them with a labeled sequence; Southern blotting detects DNA, Northern detects RNA.
In practice
A labeled probe binds only the membrane fragment complementary to it, revealing whether that sequence is present in the sample.
Where people slip
Southern blots DNA and Northern blots RNA — Western blotting, a third technique, probes proteins instead of nucleic acids.
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