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ATP Hydrolysis

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ATP hydrolysis splits off ATP's terminal phosphate, producing ADP and an inorganic phosphate ion while releasing free energy the cell can put to work.

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Hydrolyzing one mole of ATP under cellular conditions releases roughly 14 kilocalories, energy a cell's work-performing reactions draw on directly.

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ATP hydrolysis is reversible — regenerating ATP from ADP costs the cell exactly the energy hydrolysis released.