Biology / Unit 7 Bio 1563 short lesson, about 20 seconds
Physical Mapping Methods: Cytogenetic, Radiation Hybrid, and Sequence Mapping
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The idea
Physical maps are built by cytogenetic mapping of stained chromosomes, radiation hybrid mapping of fragmented DNA, or sequence mapping from DNA sequence data.
In practice
Cytogenetic mapping gives a marker's approximate chromosomal position, while sequence mapping gives its exact base-pair position.
Where people slip
The three methods trade precision for different data sources — cytogenetic mapping gives approximate position only, sequence mapping exact position.
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