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Regulation of Glycolysis by Phosphofructokinase

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Phosphofructokinase, glycolysis's rate-limiting enzyme, is allosterically inhibited by high ATP and citrate and activated by high ADP, tying pathway speed to actual energy need.

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When a cell's ATP supply is already high, phosphofructokinase slows, and glycolysis backs up rather than overproducing ATP.

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This is feedback inhibition, per Bio 0532, applied to one named enzyme — not a separate mechanism.

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