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Neuromuscular Junction and Excitation-Contraction Coupling

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At the neuromuscular junction, acetylcholine, per Bio 2827, triggers a muscle action potential that spreads inward and releases calcium, coupling nerve excitation to filament sliding.

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The action potential travels down T-tubules to calcium stores deep inside the fiber.

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The action potential does not slide filaments directly — it triggers the calcium release that does.