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Biology / Unit 6   Bio 1205 short lesson, about 20 seconds

Complementary Base Pairing

Captions available

The idea

In double-stranded DNA, adenine pairs with thymine and guanine pairs with cytosine, so a purine always faces a complementary pyrimidine across the helix.

In practice

A strand reading 5'-ATGC-3' pairs against a complementary strand reading 3'-TACG-5', matching Chargaff's measured A=T and G=C ratios.

Where people slip

Base pairing constrains which base sits opposite another — it does not fix the sequence running along either strand itself.

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