Genetic Maps from Recombination Frequency
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A genetic map places genes along a chromosome in order, using recombination frequency between pairs of genes to estimate the distance between them.
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Alfred Sturtevant, in Morgan's laboratory, used recombination frequencies to order several Drosophila genes along one chromosome in 1913.
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Map distance tracks recombination frequency, not physical DNA length — the two only estimate each other, never equal each other exactly.