Agrobacterium-Mediated Plant Transformation
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Agrobacterium tumefaciens naturally transfers DNA from its Ti plasmid into a plant's genome, a process researchers repurpose to insert chosen DNA fragments.
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Researchers remove a Ti plasmid's tumor-causing genes and replace them with a desired DNA fragment, which the bacterium then delivers into the plant genome.
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Unmodified Agrobacterium's Ti plasmid causes tumors — those genes are removed specifically for transformation.
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